Combinations of Interleukin-10 gene promoter polymorphisms with -1082A, -819T, -592A minor allele are associated with sinonasal polyposis

IL-10 gene in sinonasal polyposis

  • Sibel Ozdas Adana Science and Technology University, Faculty of Engineering, Department of Bioengineering, Adana, Turkey

Abstract

Sinonasal polyposis (SP) is an inflammatory disease involving multiple etiologies and pathogenesis. The disarray of Interleukin (IL)-10 is associated with a rised immunopathological response during the progression of many autoimmune diseases as well as the response to infection. We studied the possible role of the single nucleotide polymorphisms (SNPs) in IL-10 gene and their genotypic combinations in the SP pathogenesis. The IL-10’s promoter was genotyped in 200 participants (100 patients and 100 controls). The sites that were encompassed -1082, -819, and -592 SNP regions of extracted DNAs were analyzed by sequencing for polymorphisms. The IL-10 gene promoter polymorphisms with -1082A, -819T, -592A minor alleles, their heterozygotes and homozygotes mutant genotypes had significantly higher risks for SP (P˂ 0.05). Also, haplotype analysis demonstrated that the GTC, ACC, and GCA haplotypes in IL-10 were high-risk of SP but ATA was only in controls (P= 0.007). Also the multifactor dimensionality reduction (MDR) analysis indicated that the IL-10-1082_-819 and -1082_-819_-592 were the best predictive models for SP with 66.6% and 69% accuracy, respectively. IL-10 genotypic variations and their combinations are linked with a high-risk for the SP development in the Turkish population. The IL-10 genetic polymorphisms may lend to SP by altering the arrangement of the gene expression, affecting the severity of the inflammatory response.

Keywords:

Sinonasal polyposis; IL-10 gene; Single nucleotide polymorphisms; Interaction; Multifactor dimensionality reduction

DOI: 10.17350/HJSE19030000105

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Published
2018-04-27
How to Cite
Ozdas, S. (2018). Combinations of Interleukin-10 gene promoter polymorphisms with -1082A, -819T, -592A minor allele are associated with sinonasal polyposis. Hittite Journal of Science & Engineering, 5(4), 285-292. Retrieved from https://www.hjse.hitit.edu.tr/hjse/index.php/HJSE/article/view/HJSE19030000105
Section
SCIENCE