Review of Chromatography Methods for Purfication of Paraoxonase Enzyme


Human serum paraoxonase1 (hPON1) is a calcium dependant enzyme which has an important physiological role in organism with detoxification, antioxidant and also antiatherogenic properties. PON1 is mostly synthesized from the liver than secreted in serum with located on HDL. In order to better understand the molecular mechanism of paraoxonase 1, researchers have been increasingly interested in the development of various methods for obtaining the enzyme in pure form over the last decades. In this paper, it was reviewed several different purification and characterization methods of multifunctional enzyme paraoxonase 1 (EC


Paraoxonase1; Chromatography Methods; Enzyme Purification and Characterization

DOI: 10.17350/HJSE19030000102

Full Text: page_white_acrobat.png


Download data is not yet available.


1.Humbert R, et al. The molecular basis of the humanparaoxonase activity polymorphism. Nat Genet 1993;3:73–6

2.Aldridge WN, (1953) Serum esterase’s 2-an enzymehydrolyzing diethyl p-nitro phenyl phosphate (E600) and its identity with the A-esterase of mammalian sera. Biochem.Journal. 53, 117–124.

3.Aviram M, Rosenblat M, 2004. Paraoxonases 1, 2, and3, oxidative stress, and macrophage foam cell formationduring atherosclerosis development. Free Radic. Biol. Med.37, 1304–1316

4.Mazur, A., 1946. An enzyme in the animal organism capable of hydrolysing the phosphorus–fluorine bond of alkylfluorophosphates. Journal of Biol. Chem. 164, 271–289

5.Deakin, S.P., Bioletto, S., Bochaton-Piallat, M.L., James,R.W., 2011. HDL-associated paraoxonase-1 can redistribute to cell membranes and influence sensitivity to oxidativestress. Free Radic. Biol. Med. 50, 102–109

6.Mackness B, Durrington PN, Mackness MI, 1998 Humanserum paraoxonase. Gen. Pharmacol. 31, 329–336.

7.Primo Parma S.L., Sorenson R.C., Teiber J., La Du BN. Thehuman serum paraoxonase-arylesterase gene (PON1) is one member of a multigene family. Genomics. 1996;33:498–509.

8.La Du BN, Aviram M, Billecke S, Navab M, Primo-ParmoS, Sorenson RC, et al. On the physiological role(s) of theparaoxonases. Chem Biol Interact 1999;119–120: 379–88.

9.Sentí M, Tomás M, Fitó M, Weinbrenner T, Covas MI, Sala J,et al. Antioxidant paraoxonase 1 activity in the metabolicsyndrome. J Clin Endocrinol Metab 2003;88:5422–6.

10.Van Himbergen TM, Van Tits LJH, Roest M, Stalenhoef AFH. The story of PON1: how an organophosphate hydrolyzingenzyme is becoming a player in cardiovascular medicine.Neth J Med 2006;64(2):34–8.

11.Hegele RA. Paraoxonase genes and disease. Ann Med.1999;31: 217–224

12.Protein Purifcation. Principles, high resolution methods and applications. Third edition. Edited by J-C Jansson, 2011.Publ. John Wiley & Sons. ISBN 978-0-471-74661-4.

13.Renault F., Chabriere E., Andrieu JP , Dublet B., MassonP., Rochu D. Tandem purification of two HDL-associatedpartner proteins in human plasma, paraoxonase (PON1)and phosphate binding protein (HPBP) using hydroxyapatite chromatography Journal of Chromatography B, 836 (2006) 15–21

14 .C.E. Furlong, R.J. Richter, C. Chapline, J.W. Crabb,Biochemistry 30 (1991) 10133.

15.K N Gan, A Smolen, H W Eckerson and B N La Du Purification of human serum paraoxonase/arylesterase. Evidence forone esterase catalyzing both activities. Drug Metabolismand Disposition January 1991, 19 (1) 100-106

16.Rodrigo L, Gil F, Hernandez AF, et al. Purification andcharacterization of paraoxon hydrolase from rat liver.Biochem J 1997;321: 595–601

17.Cebeci BK, Alım Z, Beydemir Ş. In vitro effects of pesticideexposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes Turk Journal of Chem (2014) 38:512–520

18.Beydemir S, Ekinci D, Ates O. (2009). In vitro effects ofdexamethasone on human serum Paraoxonase-I (PON1)Activity. Hacettepe J Biol Chem, 37:197–205

19.Sinan S, Kockar F, Arslan O. Novel purification strategy forhumanPON1 and inhibition of the activity by cephalosporinandaminoglikozide derived antibiotics. Biochimie2006;88:565–74

20.Sayın D, Cakır DT, Gencer N, Arslan O. Effects of some metals onparaoxonase activity from shark Scyliorhinus canicula. JEnzymeInhib Med Chem 2012;27:595–8.

21.Erzengin M, Demir D, Arslan M, Sinan S. Purification andCharacterization of Paraoxonase 1 (PON1) from Swiss Black, Holstein, and Montofon Bovines Appl Biochem Biotechnol(2014) 173:1597–1606

22.Erol K, Gencer N, Arslan M, Arslan O. Purification,characterization, and investigation of in vitro inhibition bymetals of paraoxonase from different sheep breeds. ArtifCells Nanomed Biotechnol 2013;41:125–30.

23.Ekinci D, Senturk M, Beydemir S, et al. (2010). Analternativepurification of human serum paraoxonase 1and interactions withsulfonamides. Chem Biol Drug Des,76:552–8

24.Ekinci D., Beydemir Ş. Purification of PON1 from “HumanSerum and Assessment of Enzyme Kinetics Against MetalToxicity” Biol Trace Elem Res (2010) 135:112–120

25.Colak U, Gencer N. “Immobilization of paraoxonase ontochitosanan its characterization”. Artif Cells Blood SubstitImmobil Biotechnol 2012;40:290–5

26.Demir D, Gencer N, Arslan O. “An alternative purificationmethod for human serum paraoxonase 1 and its interactions with anabolic compounds”. J Enzyme Inhib Med Chem.2016;31(2):247-52.

27.Gencer N, Yavuz E. “An alternative purification methodfor human serum paraoxonase 1 and its interaction withmethidathion” Arch Physiol Biochem, 2017; 123(3): 159–164
How to Cite
Gokce, B. (2017). Review of Chromatography Methods for Purfication of Paraoxonase Enzyme. Hittite Journal of Science & Engineering, 5(4), 271-274. Retrieved from