The presence of Cronobacter sakazakii, Enterobacteriaceae spp. and Ochratoxin-A in Infant Rice-based formula and milled rice products
Rice based infant foods with reason poor hygienic conditions could be caused with fungi, opportunistic pathogens or hygiene indicator bacteria. In this study, samples of three different commercial Infant Rice-based formulae with four samples (IRF), nine milled rice (MR) samples were searched for Cronobacter sakazakii, Enterobacteriaceae spp. and Ochratoxin-A (OTA). The presence of C. sakazakii and Enterobacteriaceae spp. was detected using ISO/TS 22964/IDF/RM 210 and method, respectively. A developed method using OCHRAPREP® (REFN: A9-P14.V4) in conjunction with HPLC described by AOAC 2000.003 was used with slight modifications of the purification and HPLC conditions and with another confirmation method for OTA. The limit of quantities measurement was 1.286 μg/kg. C. sakazakii was not found in any samples. Enterobacteriaceae spp. was determined aove the legal limits (between 2.6x102 and 2.8x104 kob/g, average 3.46x104) in four samples (30.77%). OTA was detected by one IRF with milk product sample with 0.60 µg/kg (7.69%).
Khellin; Khellinone; Ammi visnaga L.; Claisen Schmidt condensation; Bis-chalcone
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