DNA-Binding Properties of Nickle (II) Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

  • Ali Arslantas Karabuk University, Department of Biomedical Engineering, Karabuk, TURKEY
  • Mehmet Salih Agirtas Yüzüncü Yıl University, Department of Chemistry, Van, TURKEY

Abstract

In this study, previously synthesized Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy ligand was chosen for its interaction with CT-DNA. CT-DNA was used to determine DNA binding properties of compound PcNi. The CT-DNA binding activities of Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy ligand was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine CT-DNA intercation of compound PcNi in Tris-HCl buffer solution. The results showed that compound PcNi binds strongly to CT-DNA via intercalation binding.

Keywords:

Uv-Vis spectroscopy; DNA-binding; Phthalocyanines; Cyclic voltametry; Electrophoresis

DOI: 10.17350/HJSE19030000054

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Research Article

Published
2017-07-21
Section
SCIENCE