DNA-Binding Properties of Nickle (II) Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement
In this study, previously synthesized Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy ligand was chosen for its interaction with CT-DNA. CT-DNA was used to determine DNA binding properties of compound PcNi. The CT-DNA binding activities of Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy ligand was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine CT-DNA intercation of compound PcNi in Tris-HCl buffer solution. The results showed that compound PcNi binds strongly to CT-DNA via intercalation binding.
Uv-Vis spectroscopy; DNA-binding; Phthalocyanines; Cyclic voltametry; Electrophoresis
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