Some Serum Oxidative Parameters in Normoglycemic Rats: Vascular Endothelial Growth Factor (VEGF) Application
VEGF has the positive effect on wound healing. In this study, the effect of VEGF application on some serum parameters during wound healing process were investigated. 36 female normoglycemic Wistar rats were used (200–250 g). Dorsolateral incisional wounds (length: 4 cm) were made on the two sides of the medulla spinalis in rats. They were divided into 3 groups: untreated group (n=12), chitosan group (n=12) and chitosan+VEGF group (n=12). The rats were sacrificed on the 3rd and 7th days of post wounding. NOx, TBARs and RSH levels were determined spectrophotometrically in serum. Results were compared by one-way ANOVA (p<0.05). Serum TBARs levels both the chitosan treated group and the VEGF application group was found decreased when compared with untreated groups (3rd and 7th days) (p <0.05). Chitosan and VEGF application were effective increasing antioxidant capacity of serum on the 7th day. Serum NOx levels decreased in the VEGF treated groups on 3rd and on 7th days (p<0.05). It can be considered that VEGF administration has a more positive systemic effect to eliminate increased oxidative damage in the serum of normoglycemic rats.
VEGF application; Chitosan; Wound healing; Oxidative stress; Antioxidant.
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