Probiotic Properties of Some Lactobacillus spp. That Can Survive in the Presence of Viral Gastroenteritis

  • Elmas Ceren Ikiz Hitit University, Department of Molecular Biology and Genetics, Çorum, Turkey
  • Gulcin Avci Hitit University, Department of Molecular Biology and Genetics, Çorum, Turkey http://orcid.org/0000-0003-4049-0756
  • Hande Alp Child Allery Specialist, Remzi Oguz Arik Neighborhood, Kavaklıdere Street, 23/2, Ankara, Turkey http://orcid.org/0000-0002-0990-7333

Abstract

Viral acute gastroenteritis is a serious health problem worldwide with the high morbidityand mortality rates. In fact there is no available antiviral treatment for gastroenteritis alternatively, the use of probiotics on which have numerous benefits, has been on the increase recently. Therefore, to be able to identify the strains that can survive in viral acute gastro-enteritis and their distinguishable features, seven strains of Lactobacillus spp. were isolated from the stool samples of 0-5 year old children with viral gastroenteritis in this study. Strains were identified by API 50 CH test. EPS production capacities, acid resistance, bile tolerance, antibiotic susceptibilities, and antimicrobial activities against Escherichia coli ATCC 25922 were determined in the order to investigate their probiotic features. Strains were character-ized as Lactobacillus plantarum. All strains survived in De Man Rogosa and Sharpe (MRS) broth with adjusted pH values of 2 and 3, despite high inhibition rates (95.2-99.2% and 98.3-99.2%, respectively). Furthermore, all strains maintained their viability within MRS broth mediums that contain 0.15%, 0.2% and 0.3% bile (viability rates as 81.4-92.5%, 80.9-87.3% and 73.2-89.2%, respectively). Exopolysaccharide production (4.13-50.33 mg/mL) was observed in all strains except for 182a. No antimicrobial activity was detected against E. coli ATCC 25922. All strains experienced high sensitivity to erythromycin while showing resistance to vancomycin. In conclusion, L. plantarum strains obtained in this study can befurther investigated for describe other probiotic features and may be used for the production of new probiotic products to provide sufficient therapies in further studies.

Keywords:

Acute Gastroenteritis, Probiotics, Lactobacillus spp.

DOI: 10.17350/HJSE19030000156

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Published
2019-12-31
How to Cite
Ikiz, E., Avci, G., & Alp, H. (2019). Probiotic Properties of Some Lactobacillus spp. That Can Survive in the Presence of Viral Gastroenteritis. Hittite Journal of Science & Engineering, 6(4), 261-267. Retrieved from https://www.hjse.hitit.edu.tr/hjse/index.php/HJSE/article/view/406
Section
SCIENCE